Figure 1.
Virulence assessment of EB-247 in insect and mouse model. (A) Survival rates of G. mellonella after the infection of different bacterial pathogens. G. mellonella were infected and observed until 7 days post infection. For each group 10 larvae were infected with ~102bacterial cfu and incubated at 37 °C. The data shown are obtained from the three independent experiments. (B) G. mellonella response to high dose infection. To observe the dose dependent virulence of EB-247, ~104 cfu were infected to G. mellonella (n = 10) and monitored for several hours post infection. (C) Intra-gastric (IG) infection of BALB/c mice (n = 4) with EB-247 and SB 300. (D) Intra-peritoneal (IP) infection of BALB/c (n = 4) with EB-247 and SB 300. For both the IG and IP route of infection, the mice were infected with 105 and 102 cfu of EB-247 and SB 300 respectively. For both routes of infection, the mice were sacrificed on day 2 post infection and the bacterial colonization was assessed in different organs. (E) Cytokines measurement of mice following intra-peritoneal infection. The blood samples from individual mice infected with IP were collected by retro orbital bleeding and cytokines were estimated using a bead-based immunoassay technique. The dotted lines in the graphs indicate minimum detection limits. ns, not significant; *statistically significant (p < 0.05, t test).