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. 2018 Mar 29;8:5402. doi: 10.1038/s41598-018-23656-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Physical characterization of OMVs secreted from RC85⁺ and RC85 cells. TEM image of OMVs released from RC85⁺ (a) and RC85 (b) cells. Bar = 100 nm. The size distribution OMVs derived from RC85⁺ (c) and RC85 (d) cells, as assessed by the Zeta-sizer. Three independent measurements were performed; means are shown with the maximum-minimum (error bars) of the percentage volume. The zeta potential of OMVs from RC85⁺ (e) and RC85 (f) cells was measured by the Zeta-sizer and each experiment was performed in triplicate. Three micrograms of whole cell lysates (WCLs), periplasmic proteins (PPs), cytoplasmic proteins (CPs), inner membrane proteins (IMPs), outer membrane proteins (OMPs) and OMVs from RC85⁺ (g) and RC85 (h) cells were separated by 12% SDS-PAGE and then the protein profiles were visualized using silver staining.