Figure 2.

Curcumin differentially alters cell cycle progression in chronic or acute myeloid leukemia cells. (A) K562 and (B) HL-60 cells were treated with curcumin 20 µM for different time points. Cells were fixed and stained with propidium iodide for analysis of cell cycle distribution. (C) K562 and (D) HL-60 cells were treated independently with 20 µM of curcumin (Cur) or with 20 µM of curcumin plus 200 nM of nocodazole (Cur + Noc) by 24 h. Cells were fixed and stained with propidium iodide for analysis of cell cycle distribution. Protein levels of cyclin B1 after the indicated treatment in (E) K562 and (F) HL-60 cells are shown. Actin was used as loading control. Control and time 0 cultures were treated with vehicle (DMSO) for 24 h. Data are representative of at least 3 independent experiments.