Figure 1.

HCPT inhibits cell viability and induces apoptosis in A549 cells. (A) Cell viability in A549 cells treated with various concentrations of (0, 50, 200 and 400 µM) HCPT using an MTT assay. (B) Expression of apoptosis-associated proteins Bcl-2 and Bax determined using western blot analysis. GAPDH (GA) was used as a loading control. The band intensities of the Bcl-2/Bax ratio were normalized to GAPDH (GA). (C) Apoptosis (Annexin⁺PI⁺ represent apoptosis) in A549 cells treated with various concentrations of (0, 50, 200 and 400 µM) HCPT assessed using Annexin V/PI staining and flow cytometry. Results are expressed as the mean ± standard deviation. In total, 10 independent experiments were performed. *P<0.05 vs. (0 µM) HCPT (control). HCPT, hydroxycamptothecin; Bcl-2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; PI, propidium iodide.