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HCPT induces autophagic activity in A549 cells. (A) Western blot analysis of expression levels of LC3I/LC3II conversion, (B) Beclin-1 and (C) p-mTOR in A549 cells treated with (0, 50, 200 and 400 µM) HCPT. Tubulin (Tu) or GAPDH (GA) was used as the loading control. Results are expressed as the mean ± standard deviation. HCPT, hydroxycamptothecin; LC3, light chain 3; p-mTOR, phosphorylated mammalian target of rapamycin; Bax. Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2.
