Table I.

Sequences of the 5′ and 3′ primers of the 4 target genes.

mRNA	Direction	Primer sequence	PCR fragment size, bp
GAPDH	Sense	5′-TGAAGGTCGGAGTCAACGGATTTGGT-3′	985
	Antisense	5′-CATGTGGGCCATGAGGTCCACCAC-3′
IL-1β	Sense	5′-ATAAGCCCACTCTACAGCT-3′	443
	Antisense	5′-ATTGGCCCTGAAAGGAGAGA-3′
IL-6	Sense	5′-GTACCCCCAGGAGAAGATTC-3′	819
	Antisense	5′-CAAACTGCATAGCCACTTTC-3′
IL-8	Sense	5′-GGCACAGTGGAACAAGGACT-3′	585
	Antisense	5′-GGCACAGTGGAACAAGGACT-3′
TNF-α	Sense	5′-TCGGGCCAATGCCCTCCTGGCCAA-3′	468
	Antisense	5′-GTAGACCTGCCCAGACTCGGCAAA-3′

IL-1β, Interleukin 1β; IL-6, interleukin-6; IL-8, interleukin-8; TNF-α, tumor necrosis factor- α.