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. 2018 Feb 23;15(5):6233–6240. doi: 10.3892/ol.2018.8113

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — H&E staining and immunohistochemical staining analysis of primary tumors (magnification, ×200) with (A) OS and (B) SQ implantation. Representative CD31 staining of primary tumors with (C) OS implantation and (D) SQ implantation. Brownish staining indicated CD31⁺ vessels. (E) Quantification of MVD as numbers of CD31⁺ vessels/field (magnification, ×100; *P<0.05). H&E, hematoxylin and eosin; CD, cluster of differentiation; OS, orthotopic implantation; SQ, subcutaneous implantation; MVD, microvessel density; HPF, high-power field.