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. 2018 Mar 23;9:601. doi: 10.3389/fimmu.2018.00601

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Copyright © 2018 Ghosn, Chamat, Prieur, Stephan, Druilhe and Bouharoun-Tayoun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-10 production induced in spleen cells from liver stage antigen 3-full length (LSA3-FL) immunized mice by peptides LSA3-GP2 and LSA3-GP8. Spleen cells of the LSA3-FL immunized humanized-spleen cells-NOD-SCID-gamma null mice were stimulated in vitro with three synthetic peptides of LSA3 (LSA3-GP2, -GP8, and -GP4). The specific production of IL-10 was measured by enzyme-linked immunospot. The specific index is the ratio of spot forming cells (SFC) of in vitro stimulated cells over SFC of non stimulated cells. Data are presented as mean values of the results obtained with each stimulating antigen using human spleen cells from five different donors. Each dot corresponds to the result obtained with spleen cells pooled from mice of one experimental group.