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. 2018 Mar 5;18(3):784. doi: 10.3390/s18030784

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescent images and amounts of immobilized His-tagged GFP and DsRed by applying AC electric field. The conditions of applying AC electric field are described as follows: 10, 20, 40, 60, and 80 Hz at the constant applied voltage of 80 Vpp for the duration time of 30 min. (A) After the application of AC electric field, fluorescence images of immobilized His-tagged GFP. (B) After Milli-Q water wash and imidazole treatment, amounts of immobilized His-tagged GFP. (C) After the application of AC electric field, fluorescence images of immobilized His-tagged DsRed. (D) After Milli-Q water wash and imidazole treatment, amounts of immobilized His-tagged DsRed. Open and closed circle denote the amounts of immobilized His-tagged GFP and DsRed after Milli-Q water wash and imidazole treatment, respectively. Error bars represent the standard deviations from three independent experiments. Scale bar = 1 mm.