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. 2018 Mar 5;18(3):784. doi: 10.3390/s18030784

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fluorescent images and amounts of immobilized His-tagged GFP and DsRed by applying AC electric field. The conditions of applying AC electric field are described as follows: 0, 20, 40, 60, 80, 100, and 120 Vpp of 20 Hz for the duration time of 30 min. (A) After the application of AC electric field, fluorescence images of immobilized His-tagged GFP. (B) After Milli-Q water wash and imidazole treatment, amounts of immobilized His-tagged GFP. (C) After the application of AC electric field, fluorescence images of immobilized His-tagged DsRed. (D) After Milli-Q water wash and imidazole treatment, amounts of immobilized His-tagged DsRed. Open and closed circle denote the amounts of immobilized His-tagged GFP and DsRed after Milli-Q water wash and imidazole treatment, respectively. Error bars represent the standard deviations from three independent experiments. Scale bar = 1 mm.