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. 2018 Mar 5;18(3):784. doi: 10.3390/s18030784

Figure 6.

Figure 6

Histogram of amounts of immobilized His-tagged GFP at a pH 5.0, 5.6, and 8.0. To prevent an aggregation of His-tagged GFP due to the acidic condition, this experiment shortened the duration time of the application of AC electric field. The conditions of applying AC electric field are described as follows: 20 Hz at the constant applied voltage of 80 Vpp for the duration time of 10 min. White bars and black bars indicate the amounts of His-tagged GFP immobilized on the substrate surfaces after wash buffer and imidazole treatment, respectively. Error bars represent the standard deviations from three independent experiments.