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. 2001 Sep 11;98(20):11545–11550. doi: 10.1073/pnas.191112198

Figure 3.

Figure 3

The identification of VEGF-specific antoantibody in sera in Western blot analysis. Recombinant Xenopus VEGF, VEGF 120, 164, 188, and 165 as well as other growth factors (bFGF and PlGF) were stained with sera isolated from mice immunized with XVEGF-p and other control sera. The positive bands for VEGF can be recognized with the sera isolated from mice immunized with XVEGF-p (A), but negative staining from mice immunized with MVEGF-p (B) or other control groups. (C) Positive staining for bFGF and PlGF was found with the antibodies against bFGF and PlGF, respectively, as positive controls for A, but (D) negative staining was found with control IgG.