Figure 2.

The BARF1 promoter reporter construct is transactivated by the ΔNp63α isoform. (A) Representative graphs of a cotransfection of the reporter construct with individual p63 isoforms in AGS and (B) SNU-719 cells. Only the ΔNp63α isoform shows transcriptional activity in both cell lines; (C) Representative SDS-page Westernblot of both the TAp63α and ΔNp63α isoform demonstrates comparable protein levels; (D) Methylation of the BARF1 promoter reduces relative luciferase activity induced by ΔNp63α, representative graph; (E) Luciferase activity is 28 fold upregulated by ΔNp63α and not by TAp63α (n = 7). Fold induction induced by ΔNp63α compared with constitutive activity of the methylated promoter is unchanged (29 fold) (n = 3).