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. 2018 Mar 17;10(3):76. doi: 10.3390/cancers10030076

Figure 4.

Figure 4

Multiple p63 response elements are responsible for BARF1 promoter transactivation. (A) Potential p53 family responsive elements are depicted on the BARF1 promoter region (boxes). Black vertical lines represent methylation sites. Rounded grey indicators point to the deletion mutants made from the BARF1 reporter construct, shortening the BARF1 promoter sequence from the original −679 to −63 relative to the ATG start site; (B) AGS cells were transfected with the deletion mutant luciferase constructs and with or without ΔNp63α expression vector. The ΔNp63α induced luciferase activity (fold) was measured 48 hours after transfection. A representative experiment is shown; (C) 293RKO cells demonstrate that, unlike R, ΔNp63α has only minor transactivating activity (3 fold) of BARF1 in the context of the intact viral genome; (D) Endogenous p63 levels as detected by Western blot, HEK293 cells stably expressing either TAp63 or ΔNp63 were used as positive control.