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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Kidney Int. 2018 Mar 23;93(5):1240–1246. doi: 10.1016/j.kint.2018.01.014

Figure 2. CoRL-PODO mice specifically and inducibly label renin expressing cells, and constitutively label nephrin expressing cells (podocytes).

Figure 2

Ren1cCreER/tdTomato mice were crossed with Nphs1-FLPo/FRT-EGFP mice to generate dual-reporting Ren1cCreER/tdTomato/Nphs1-FLPo/FRT-EGFP mice. Immunohistochemistry for Collagen IV is used to easily identify glomeruli in panels A, C, D and F. Higher magnification images of the areas of interest outlined by the white boxes are shown to the right in superscript 1–4 for green, red, far red and merged fluorescence channels respectively.

A) Confocal direct fluorescence of representative CoRL-PODO mice administered tamoxifen, express tdTomato in CoRL in the JGC (arrow) and EGFP in podocytes in the glomerular tuft. B) Immunohistochemistry for renin in tamoxifen-induced CoRL-PODO mice, shows co-labeling of renin (blue) and tdTomato in CoRL in the JGC (purple color, arrow). C) When CoRL-PODO mice are given the vehicle corn oil, there is no induced expression of tdTomato (arrow). The constitutive Nphs1-FLPo/FRT-EGFP transgene allows for the expression of EGFP. D) In CoRL-PODO mice given tamoxifen, that lack the Ren1c-CreER transgene, there is no inducible labeling of tdTomato CoRL (arrow) but the constitutive Nphs1-FLPo/FRT-EGFP transgene allows for the expression of EGFP. E) Immunohistochemistry for the podocyte protein nephrin in tamoxifen induced CoRL-PODO mice shows co-labeling of nephrin and EGFP in podocytes (arrow). F) In CoRL-PODO mice given tamoxifen which do not have the Nphs1-FLPo transgene, there is no labeling of EGFP in podocytes, but the induction of the Ren1c-CreER transgene allows for the expression of tdTomato in CoRL in the JGC (arrow). Scale bars = 25μm.