Figure A5.

Growth and viability curves for prolonged batch cultivations for the production of BR021 in the cell line S2 Mt-BR021-V5/His E3. After entering the stationary phase and the depletion of essential nutrients, the cell population lost viability. The transition from an active to a decaying culture is visible in the corresponding Δε-OD₈₈₀ phase trajectories. Black dots represent the process before 120 h, followed by gray dots for the subsequent time points. SDS PAGE shows that the lower cell viability is associated with protein degradation and the release of host cell protein. The shifted band position results from the exceptional high pI of 10.72 that alters the migration properties of BR021 during SDS PAGE.