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. 2001 Sep 11;98(20):11563–11568. doi: 10.1073/pnas.201167798

Figure 2.

Figure 2

Analysis of prostate cancers from Pten+/− TRAMP mice with loss of the WT allele. (A) DNAs from tails or primary prostate cancers were extracted, digested with SacI, and analyzed by Southern blotting with a Pten probe that distinguishes the WT (12 kb) and KO (5 kb) alleles. Tails 1 and 2 and tumors 1, 3, 4, and 7 were from Pten+/− TRAMP mice, whereas tails 3 and 4 and tumors 2, 5, 6, and 8 were from WT TRAMP mice. (B) DNAs were extracted from tails or primary prostate cancers, then analyzed by PCR with primers amplifying the D19Mit119 polymorphic locus, followed by agarose gel electrophoresis. The allele derived from the SV129 chromosome gives a 281-bp band, whereas the allele from the C57BL/6 chromosome gives a 265-bp band. c, water control; Tails 1–3, tail DNAs: Tumors 1–6, prostate cancers with loss of the WT Pten allele by Southern blotting.