Skip to main content
. 2001 Sep 11;98(20):11563–11568. doi: 10.1073/pnas.201167798

Figure 3.

Figure 3

Analysis of prostate cancers from Pten+/− TRAMP mice that retain the WT Pten allele. (A) DNAs were extracted from primary prostate cancers from Pten+/− TRAMP mice and then analyzed by PCR with primers amplifying the D19Mit119 polymorphic locus followed by agarose gel electrophoresis. All tumors retained the WT Pten allele as determined by Southern blotting. The allele derived from the SV129 chromosome gives a 281-bp band, whereas the allele from the C57BL/6 (WT) chromosome gives a 265-bp band. (B) Western blot analysis with anti-Pten antibody and anti-β-actin (control) antibody was performed on protein extracts from primary prostate cancers arising in the Pten+/− TRAMP mice with retention of the WT Pten allele as determined by Southern blotting (Het tumors 1–5), a primary prostate cancer from a WT TRAMP mouse, and the human prostate cancer cell lines PC3, known to have a homozygous deletion of PTEN (PC).