Figure 4.

Analysis of prostate cancers from WT TRAMP mice. (A) DNAs were extracted from primary prostate cancers arising in WT TRAMP mice, then analyzed by PCR with primers amplifying the D19Mit119 polymorphic locus, followed by agarose gel electrophoresis. The allele derived from the SV129 chromosome gives a 281-bp band, whereas the allele from the C57BL/6 chromosome gives a 265- bp band. (B) Western blot analysis with anti-Pten antibody and anti-β- actin (control) antibody was performed on protein extracts from primary prostate cancers arising in WT TRAMP mice. WT 1 and 2 were from tumors without LOH at the D19Mit119 polymorphic locus, whereas WT LOH 1–5 were from tumors with LOH at this locus.