Table 2.
Treatments (mg/L) | Times (h) | Amount of Estrogens | |||||
---|---|---|---|---|---|---|---|
Residual (μg·L−1) | Extracellular (10−8 µg·cell−1) |
Intracellular (10−8 µg·cell−1) | |||||
E2 | DES | E2 | DES | E2 | DES | ||
0.1 | 24 | 29.2 ± 0.7 * | 41.2 ± 1.1 | 0.31 ± 0.08 | 0.45 ± 0.15 | 0.21 ± 0.05 | 0.23 ± 0.08 |
48 | 24.3 ± 1.4 * | 34.3 ± 1.8 | 0.25 ± 0.02 * | 0.85 ± 0.05 | 0.20 ± 0.08 * | 0.43 ± 0.05 | |
96 | 17.6 ± 1.7 | 9.9 ± 0.8 * | 0.31 ± 0.05 | 0.24 ± 0.05 | ND * | 0.15 ± 0.07 | |
0.5 | 24 | 213.1 ± 5.5 * | 268.7 ± 23.1 | 0.98 ± 0.24 * | 6.0 ± 0.25 | 1.45 ± 0.33 * | 2.12 ± 0.42 |
48 | 121.7 ± 11.5 * | 158.7 ± 13.3 | 0.88 ± 0.1 * | 2.11 ± 0.67 | 2.26 ± 0.12 * | 4.34 ± 0.15 | |
96 | 95.8 ± 10.6 * | 129.5 ± 11.4 | 0.45 ± 0.11 * | 3.33 ± 0.59 | 1.6 ± 0.21 * | 4.10 ± 0.15 | |
1.5 | 24 | 750.2 ± 18.1 * | 861.9 ± 10.6 | 14.8 ± 6.6 * | 18.1 ± 3.9 | 8.41 ± 2.1 * | 14.2 ± 5.51 |
48 | 501.5 ± 11.9 * | 778.1 ± 15.5 | 7.89 ± 0.93 * | 14.1 ± 0.75 | 6.32 ± 0.24 * | 18.2 ± 0.51 | |
96 | 377.5 ± 16.7 * | 680.3 ± 14.2 | 1.3 ± 0.37 * | 26.52 ± 1.25 | 2.8 ± 0.25 * | 24.2 ± 2.83 |
Mean and standard deviation of three replicates are shown. Asterisk (*) indicates value significantly different between the amount of E2 and DES in the same treatment (concentration and time) (p < 0.05).