(A) The Igf2-P0 consensus sequence is aligned with three DNA sequences used for binding assays (mouse Igf2-P0, chimp Igf2-P0, and control).
(B) Binding affinities of mZFP568 against the three oligos.
(C) A 12% SDS-PAGE showing purified ZF proteins used in the study.
(D) The binding of five ZF fragments against mIgf2-P0 sequence.
(E) A model of ZF1-10 (colored) binding mIgf2-P0. ZF1 (circled) is involved in intra-molecular interactions between ZF2 and ZF7.
(F–G) ZF2 (F) and ZF4 (G) involvement in DNA phosphate backbone interactions at the AT-rich stretch.