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. Author manuscript; available in PMC: 2019 Mar 22.
Published in final edited form as: Cell. 2018 Mar 15;173(1):221–233.e12. doi: 10.1016/j.cell.2018.02.058

Figure 2. mZFP568 binds Igf2-P0 sequence.

Figure 2

(A) The Igf2-P0 consensus sequence is aligned with three DNA sequences used for binding assays (mouse Igf2-P0, chimp Igf2-P0, and control).

(B) Binding affinities of mZFP568 against the three oligos.

(C) A 12% SDS-PAGE showing purified ZF proteins used in the study.

(D) The binding of five ZF fragments against mIgf2-P0 sequence.

(E) A model of ZF1-10 (colored) binding mIgf2-P0. ZF1 (circled) is involved in intra-molecular interactions between ZF2 and ZF7.

(F–G) ZF2 (F) and ZF4 (G) involvement in DNA phosphate backbone interactions at the AT-rich stretch.