Table 1.
Log numbers of CFU (data are log means±standard error [SE], n=3) of culturable rhizobacteria in different compartments of barley roots, compared to free soil, developed on various culture media; and bacterial quantification using qPCR of surface-sterilized barley roots. The mean value of qPCR cell numbers (obtained indirectly assuming that the average 16S rRNA gene copy number per cell is 3.6) is log 9.37±0.001 g−1 root dry weight obtained for four replicates.
| Culture media | Endo-rhizosphere Log CFUs±SE (% of culturability) |
Ecto-rhizosphere Log CFUs±SE |
Free soil Log CFUs±SE |
|---|---|---|---|
| Nutrient agar | 7.91±0.01c (3.4) | 8.19±0.11d | 6.19±0.05b |
| CCM | 7.96±0.09c (3.9) | 7.97±0.06d | 6.16±0.03b |
| Soil extract | 7.96±0.03bc (3.8) | 9.79±0.06a | 6.20±0.02b |
| Turfgrass powder | 8.59±0.09a (16.3) | 8.77±0.17bc | 6.75±0.17a |
| Clover powder | 7.95±0.08c (3.8) | 7.89±0.03d | 6.24±0.16b |
| Clover slurry | 7.89±0.02c (3.3) | 8.77±0.18c | 5.48±0.18c |
| Cactus powder | 8.16±0.08b (6.1) | 9.22±0.17b | 6.00±0.03b |
| Cactus slurry | 8.03±0.01bc (4.5) | 8.73±0.03c | 6.08±0.05b |
| LSD (P<0.05) | 0.1918 | 0.3797 | 0.3414 |
*
Different letters indicate different significance levels.