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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Cancer Res. 2017 Apr 28;77(13):3655–3665. doi: 10.1158/0008-5472.CAN-16-3199

Fig. 2. The CXCL1-CXCR2 axis is required for recruitment of MDSCs into pre-metastatic liver and liver metastasis.

Fig. 2

(A) The protein levels of human CXCL1 in the cell culture supernatants of indicated cells. (B–D) The protein levels of human and/or mouse CXCL1 in cecal tumor (B), pre-metastatic livers (C), and blood (D) of NSG mice injected with indicated cells as described in Fig. 1A–C. (E) The protein levels of mouse CXCL1 in normal colonic tissues and liver of WT mice and colonic tumors and liver of Apc−/−/KrasG12D mice. (F) Data represents the percentage of CXCR2-positive gMDSCs in total gMDSCs from bone marrow (BM) and peripheral blood taken from NSG mice injected with HCoEpiC cells, HCT-116 cells (left panel), or LS-174T cells (right panel) as described above. (G) The numbers of DCs, gMDSCs, and macrophages (Mϕ) in pre-metastatic livers of NSG mice treated with vehicle or SB225002 after cecal injection of HCT-116 cells. (H) Gross view of liver metastatic tumors (left panels) and the average numbers of liver metastatic tumors at different size and total that includes all sizes (right panel) in NSG mice treated with vehicle or SB225002 after cecal injection of HCT-116 cells. The error bar indicates ± SEM. *p<0.05.