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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Orthop Res. 2017 Nov 22;36(2):699–710. doi: 10.1002/jor.23779

Figure 2. Histological assessment of osteophyte and fracture callus size and ossification.

Figure 2

(A) Representative H&E stained images for fracture calluses and chondro/osteophytes at 7, 14, 21, and 28 days post-injury (FC= fracture callus; F= femur; M= meniscus; T= tibia). Scale bar is 1 mm. Fracture callus and chondro/osteophyte areas outlined in black. (B) Fracture callus and chondro/osteophyte areas as a function of days after injury. Fracture callus growth occurred at earlier time points, with remodeling and a subsequent decrease in area at later time points. Osteophyte growth occurred at comparatively later time points, but continued throughout the duration of the experiment. A significant interaction between time point and injury type indicates statistical differences in growth trend (p=0.0002). (C) Ossification percentage for fracture calluses and chondro/osteophytes over time. Fracture calluses exhibit earlier ossification, eventually plateauing by days 21–28. Osteophyte ossification continuously increased started at day 14 after injury. Time points that do not share a letter are significantly different from each other (p<0.05). Data is presented as mean ± SD.