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. 2001 Aug 14;98(20):11598–11603. doi: 10.1073/pnas.181181198

Figure 3.

Figure 3

Localization of Mdm2 characterized by confocal microscopy. (a) Endogenous Mdm2 in serum-starved MCF-7 cells transfected with constitutively active Akt (CAAkt) or incubated in the absence or presence of LY294002 and then treated with IGF-1 (5 min). (b) HEK 293 cells transiently transfected with human Flag-Mdm2 and Flag-Mdm2, then treated with wortmannin (1 h), Flag-Mdm2 and KD-Akt, Flag-Mdm2 and Δp85 PI3-kinase, and Flag-Mdm2 and KD-Akt, and then treated with IGF-1. (c) Primary, serum-starved human keratinocytes were treated with growth factor-free medium (control) or medium containing IGF-1. Keratinocytes were also transfected with Flag-Mdm2, Flag-Mdm2, and KD-Akt, or treated with LY294002 (1 h). (d) HEK 293 cells transfected with Flag-Mdm2, Flag-S166A, Flag-186A, and Flag-2xSA. Additionally, cells were cotransfected with Flag-2xSA and CA-Akt or were transfected with Flag-2xSA and then treated with IGF-1.