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. 2018 Feb 26;19(3):653. doi: 10.3390/ijms19030653

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Bacopaside II increased endothelial cell apoptosis as determined by flow cytometry. Histograms (left) show percentages of apoptotic cells (early and late) in a representative experiment for data shown in the scatter plots at the different concentrations of bacopaside II. Results are shown for 2H11 (A,B), 3B11 (C,D) and HUVEC (E,F). In (A,C,E), results shown as the mean ± SD; in (B,D,F), scatter plots show population gates of viable cells (left lower quadrant), or cells either in early apoptosis (right lower quadrant), late apoptosis (right upper quadrant), or necrosis (left upper quadrant).