Table 1.

Component loadings and mean PC scores showing the association between total anthocyanin and expressions of regulatory and biosynthetic genes as determined by Principal Component Analysis. PC, Principal Component; p, statistical significance; SD, Standard Deviation.

Variable		PC1	PC2	PC3
Regulatory Genes
MYB	FaMYB11	−0.080	0.128	−0.115
	FaMYB1	0.132	−0.020	0.139
	FaMYB10	0.260	−0.094	0.044
	FaMYB5	0.205	0.146	0.131
	FaMYB9	−0.012	0.253	−0.271
	FaEOBII	0.011	−0.216	0.151
bHLH	FabHLH33	−0.173	0.142	−0.043
	FabHLH3-delta	0.128	0.192	0.232
	FabHLH3	0.148	0.170	0.238
	FabHLH1	0.018	0.271	0.267
WD40	FaWD40-1	−0.104	−0.110	0.166
	FaWD-1	−0.033	0.068	0.239
	FaTTG1	0.108	0.154	0.285
	FaWD44-1	−0.176	0.198	−0.066
Early Biosynthetic Genes
Phenylalanine	FaPAL1	0.064	0.171	−0.264
	FaPAL2	0.189	0.078	−0.254
Cinnamic acid	FaC4H	−0.047	0.228	−0.113
	FaCC1	0.143	−0.059	0.026
Coumaroyl CoA	Fa4CL7	0.268	−0.094	0.036
	Fa4CL2	−0.204	−0.116	−0.104
Chalcone	FaCHI3	0.266	0.051	0.022
	FaCHI1	0.254	−0.014	−0.121
	FaCHS	0.256	−0.100	−0.074
Flavanone	FaFLS	0.018	0.273	0.134
	FaF3H	0.258	0.028	−0.137
Late Biosynthetic Genes
Dihydroflavonol	FaDFR4-1	−0.035	0.294	0.222
	FaDFR4-2	−0.136	0.014	0.216
	FaDFR4-3	0.264	0.042	−0.076
Leucoanthocyanidin	FaLDOX	0.266	0.059	0.037
	FaANR	−0.049	0.233	−0.265
	FaLAR	0.074	0.300	−0.157
Anthocyanidin	FaUFGT1	0.260	−0.015	0.126
	FaUFGT2	0.082	0.245	−0.192
Total anthocyanin		0.229	−0.133	−0.110
Eigenvalue		12.809	6.630	6.280
% variation explained		36.6	18.9	17.9
P (Genotype × Fruit developmental stage)		<0.001	<0.001	<0.001
Genotype × Fruit Developmental Stage		Mean PC Scores ± SD
Tokun	Green	−2.75 ± 0.01 g	−0.06 ± 0.01 d	−2.08±0.01 e
	White	−3.15 ± 0.37 g	−2.48 ± 0.02 e	1.87±0.10 b
	Ripe	−1.23 ± 0.14 e	−3.51 ± 0.08 f	−0.45±0.12 cd
Maehyang	Green	−2.64 ± 0.17 fg	0.55 ± 0.26 d	0.04±0.13 c
	White	−2.18 ± 0.30 f	1.54 ± 0.02 c	1.11±0.16 b
	Ripe	3.57 ± 0.06 b	−3.44 ± 0.04 f	−1.10±0.02 de
Soelhyang	Green	−0.66 ± 0.19 d	3.94 ± 0.55 a	−4.26±0.41 f
	White	0.89 ± 0.13 c	2.88 ± 0.30 b	5.01±0.67 a
	Ripe	8.14 ± 0.02 a	0.588 ± 0.36 d	−0.14±0.64 cd

PAL, Phenylalanine ammonia lyase; C4H, cinnamate-4-hydroxylase; 4CL, 4-coumaroyl-CoA-ligase; CHS, Chalcone synthase; CHI, Chalcone isomerase; F3H, Flavanone 3 hydroxylase; FLS, Flavonol synthase; DFR, Dihydroflavanol reductase; LAR, Leucoanthocyanidin reductase; ANR, Anthocyanidin reductase; LDOX, leucoanthocyanidin dioxygenase; UFGT, uridine diphosphate-glucose:flavonoid 3-O-glucosyltransferase. Statistically significant differences in the relative expressions are indicated by different letters as per Tukey’s pairwise comparisons.