Figure 7.

M-CTX-Fc-L-Dox exhibited the lowest inhibition concentration (IC₅₀) and shortest exposure time (IT₅₀) in U251MG-P1 cells. In vitro cytotoxicity IC₅₀ of doxorubicin in different formulations after 72 h of exposure to U251MG-P1 and SK-BR-3 cells was evaluated and compared (top). IT₅₀ with doxorubicin at IC₁₀₀ were evaluated and compared (bottom). The data presented as the mean ± SD (n = 3). The statistical significance in mean values of more than two groups was determined using one-way analysis of variance (ANOVA) and post hoc Tukey HSD were applied using M-CTX-Fc-L-Dox liposome as control. *, p < 0.05; **, p < 0.01; ***, p < 0.005; NSD, no significant difference; #, p < 0.05 versus Dox.