Table 4.
Antibodies used for western blot assays. Primary and peroxidase-conjugated secondary antibodies. Catalogue numbers are shown between brackets.
| Primary Antibody | Dilution | Company |
| Total TH monoclonal (mouse) | 1:2000 | Sigma, St. Louis, MO, USA (T1299) |
| Ser19p TH polyclonal (rabbit) | 1:4000 | Chemicon, Millipore Co., Temecula, CA, USA (AB5425) |
| Ser31p TH polyclonal (rabbit) | 1:750 | Chemicon, Millipore Co., Temecula, CA, USA (AB5423) |
| Ser40p TH polyclonal (rabbit) | 1:2000 | Chemicon, Millipore Co., Temecula, CA, USA (AB5935) |
| β-actin monoclonal (mouse) | 1:1000 | Sigma, St. Louis, MO, USA (A2066) |
| Secondary Antibody | Dilution | Company |
| Anti-mouse (Goat) (for Total TH) | 1:5000 | Santa Cruz, Dallas, TX, USA (sc2005) |
| Anti-rabbit (Goat) (for Ser19p TH) | 1:7500 | Chemicon, Millipore Co., Temecula, CA, USA (AP307P) |
| Anti-rabbit (Goat) (for Ser31p TH) | 1:1500 | Chemicon, Millipore Co., Temecula, CA, USA (AP307P) |
| Anti-rabbit (Goat) (for Ser40p TH) | 1:5000 | Chemicon, Millipore Co., Temecula, CA, USA (AP307P) |
| Anti-mouse (Goat) (for β-actin) | 1:4000 | Santa Cruz, Dallas, TX, USA (sc2005) |