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Comparison of published protocols used to isolate neuronal exosomes from blood and the method implemented in the present study. Preliminary studies revealed 2 steps (see red text) which critically influenced the production and analysis of neuronal exosomes: (i) the use of recombinant thrombin instead of thromboplastin D, and (ii) an increased amount of biotinylated anti-L1CAM and longer incubation time with streptavidin resin.
