Table 2.
List of proteins differently expressed in ‘T337’ basal cuttings with IBA treatment and control.
| Accession No. a | Description | SwissProt Accession b | Identity (%) c | %COV (95) d | Unique Peptides | Ratio e |
|---|---|---|---|---|---|---|
| Phytohormone signaling | ||||||
| MDP0000873893 | Indole-3-acetic acid-amido synthetase | O82333 | 80.27 | 5.7 | 1 | 2.03 |
| MDP0000121609 | Indole-3-acetic acid-amido synthetase | O82333 | 80.14 | 17 | 2 | 1.64 |
| MDP0000310711 | IAA-amino acid hydrolase ILR1 | P54968 | 54.53 | 4.2 | 2 | 1.74 |
| MDP0000663451 | IAA-amino acid hydrolase ILR1 | P54968 | 62.83 | 10 | 3 | 1.52 |
| MDP0000290695 | Indole-3-acetate O-methyltransferase 1 | Q9FLN8 | 73.58 | 1.8 | 1 | 0.43 |
| MDP0000140463 | ADP-ribosylation factor gtpase-activating protein | O80925 | 70.28 | 6.8 | 3 | 0.67 |
| MDP0000250432 | ADP-ribosylation factor gtpase-activating protein | O80925 | 54.84 | 1.4 | 1 | 0.58 |
| MDP0000288128 | ADP-ribosylation factor gtpase-activating protein | O80925 | 62.68 | 1.3 | 1 | 0.37 |
| MDP0000186518 | Histidine-containing phosphotransfer protein 3 | Q9SAZ5 | 56.1 | 6.1 | 1 | 0.32 |
| MDP0000509768 | Arabidopsis response regulator ARR9 | O80366 | 59.18 | 5.7 | 1 | 0.45 |
| MDP0000212178 | Arabidopsis response regulator ARR5 | Q9ZWS6 | 78.01 | 4 | 1 | 0.53 |
| MDP0000250737 | Arabidopsis response regulator ARR3 | Q9ZWS9 | 76.61 | 5.9 | 2 | 0.29 |
| MDP0000846313 | Arabidopsis response regulator ARR1 | Q940D0 | 63.64 | 5.7 | 1 | 0.65 |
| MDP0000175691 | 1-aminocyclopropane-1-carboxylate oxidase 1 | Q9LSW7 | 29.34 | 6 | 1 | 1.57 |
| MDP0000663852 | 1-aminocyclopropane-1-carboxylate oxidase 1 | Q0WPW4 | 46.59 | 4.8 | 1 | 1.6 |
| MDP0000839921 | 1-aminocyclopropane-1-carboxylate oxidase 1 | Q84MB3 | 47.74 | 7 | 2 | 1.81 |
| MDP0000195885 | 1-aminocyclopropane-1-carboxylate oxidase 1 | Q00985 | 100 | 7.6 | 2 | 0.29 |
| MDP0000324718 | Ethylene-responsive transcription factor 4 | O80340 | 74.71 | 5.6 | 1 | 0.57 |
| MDP0000284624 | Abscisic acid receptor PYL9 | Q84MC7 | 81.29 | 6 | 1 | 1.56 |
| MDP0000215106 | Abscisic acid-insensitive 5-like protein 2 | Q9LES3 | 52.22 | 3.2 | 1 | 0.51 |
| MDP0000228470 | Abscisic acid receptor PYL4 | O80920 | 80.12 | 4.6 | 1 | 0.58 |
| Carbohydrate metabolism and energy production | ||||||
| MDP0000866748 | Phosphoglucomutase | Q9SCY0 | 84.31 | 14.9 | 7 | 1.79 |
| MDP0000256619 | ADP-glucose pyrophosphorylase 1 | P52417 | 88.72 | 16.7 | 1 | 2.44 |
| MDP0000298815 | α-amylase | P17859 | 72.8 | 2.2 | 2 | 2.2 |
| MDP0000657082 | α-mannosidase | P34098 | 61.35 | 9.1 | 2 | 1.84 |
| MDP0000095637 | Granule-bound starch synthase 1 | O82627 | 74.56 | 21.2 | 9 | 2.69 |
| MDP0000133306 | d-sorbitol-6-phosphate dehydrogenase | P28475 | 84.52 | 11.9 | 1 | 2.62 |
| MDP0000661960 | Xyloglucan endotransglucosylase/hydrolase protein 6 | Q8LF99 | 78.36 | 14.4 | 2 | 2.7 |
| MDP0000129346 | α-1,4 glucan phosphorylase L isozyme | P53536 | 81.22 | 21.4 | 15 | 1.63 |
| MDP0000320017 | Xyloglucan endotransglucosylase/hydrolase protein | Q38910 | 75.76 | 2.8 | 1 | 1.5 |
| MDP0000296747 | α-glucosidase | Q9F234 | 45.19 | 8.7 | 5 | 1.5 |
| MDP0000177786 | 1,4-α-glucan-branching enzyme | P30924 | 73.7 | 10.7 | 7 | 1.5 |
| MDP0000202465 | β-galactosidase | Q9FN08 | 60.98 | 19 | 13 | 0.19 |
| MDP0000290090 | β-galactosidase 6 | Q10NX8 | 42.16 | 4.8 | 1 | 0.47 |
| MDP0000295518 | α-l-fucosidase 1 | Q8GW72 | 71.43 | 10.7 | 4 | 1.67 |
| MDP0000863563 | β-galactosidase 9 | Q9SCV3 | 77.39 | 16.6 | 5 | 1.71 |
| MDP0000237069 | α-galactosidase | Q9FXT4 | 25.52 | 8.4 | 2 | 1.71 |
| MDP0000823956 | Hexokinase | Q9SEK2 | 78.16 | 13.7 | 3 | 1.65 |
| MDP0000294262 | 6-phosphofructokinase | Q41141 | 82.18 | 8.1 | 1 | 0.4 |
| MDP0000254412 | 6-phosphofructokinase | Q8VYN6 | 79.88 | 5.5 | 1 | 1.55 |
| MDP0000835914 | Glyceraldehyde-3-phosphate dehydrogenase | P12859 | 88.47 | 16.2 | 5 | 1.62 |
| MDP0000527995 | Glyceraldehyde-3-phosphate dehydrogenase | P12858 | 88.15 | 24.8 | 6 | 1.69 |
| MDP0000543856 | Glyceraldehyde-3-phosphate dehydrogenase | Q8S0G4 | 79.87 | 6.5 | 1 | 1.84 |
| MDP0000174843 | 3-phosphoglycerate kinase | Q42961 | 88.13 | 22.2 | 1 | 1.63 |
| MDP0000212948 | 3-phosphoglycerate kinase | Q42961 | 84.14 | 23.8 | 1 | 0.57 |
| MDP0000211987 | Aldehyde dehydrogenase | Q9ZPB7 | 82.41 | 8.7 | 4 | 1.57 |
| MDP0000221713 | Aldehyde dehydrogenase | Q9SU63 | 82.05 | 18.4 | 7 | 2 |
| MDP0000236430 | Alcohol dehydrogenases | P42734 | 66.77 | 8.6 | 4 | 1.78 |
| MDP0000267169 | Fructose-1,6-bisphosphatase | P46283 | 75.31 | 4.9 | 1 | 2.07 |
| MDP0000273014 | Fructose-1,6-bisphosphatase | P46275 | 78.87 | 19.5 | 6 | 1.67 |
| MDP0000244771 | Fructose-1,6-bisphosphatase | P46283 | 83.72 | 5.8 | 3 | 1.57 |
| MDP0000277811 | Fructose-1,6-bisphosphatase | P46283 | 80.67 | 5 | 1 | 1.95 |
| MDP0000275261 | Probable fructokinase-2 | Q9LNE3 | 65.8 | 8.5 | 1 | 1.57 |
| MDP0000178814 | Pyruvate dehydrogenase complex | Q54M22 | 53.72 | 6.3 | 1 | 1.59 |
| MDP0000325085 | Isocitrate dehydrogenase | P50217 | 89.78 | 21.1 | 3 | 1.54 |
| MDP0000214399 | 2-oxoglutarate and Fe(II)-dependent oxygenase superfamily protein | Q39110 | 26.8 | 3.2 | 1 | 1.68 |
| MDP0000384593 | Malic enzyme | P12628 | 83.78 | 23.7 | 8 | 1.58 |
| MDP0000221561 | Malic enzyme | P51615 | 83.94 | 24.4 | 7 | 1.5 |
| MDP0000313179 | NADH: ubiquinone oxidoreductase 20 kd subunit | P42027 | 98.11 | 5.5 | 1 | 1.53 |
| MDP0000362465 | Cytochrome b-c1 complex subunit 9 | P46270 | 76.39 | 22.4 | 2 | 1.57 |
| MDP0000124616 | Cytochrome c oxidase subunit 5C-2 | Q9LZQ0 | 77.78 | 12.7 | 1 | 1.65 |
| MDP0000263444 | Cytochrome c oxidase subunit 6b-1 | Q9S7L9 | 84.06 | 4.5 | 1 | 0.41 |
| MDP0000385730 | ATP synthase gamma chain, chloroplastic | P29790 | 85.68 | 9.7 | 3 | 1.75 |
| MDP0000168167 | Atpases with chaperone activity, chloroplastic | Q9LF37 | 81.33 | 16.6 | 2 | 1.73 |
| MDP0000929055 | ATP synthase subunit alpha, mitochondrial | P05495 | 99.16 | 10.1 | 2 | 1.65 |
| MDP0000360515 | ATP synthase subunit beta, mitochondrial | P17614 | 93.16 | 38.9 | 2 | 1.59 |
| MDP0000785964 | ATP synthase subunit delta, mitochondrial | Q40089 | 92.11 | 13.9 | 1 | 0.41 |
| MDP0000624197 | ATP synthase subunit delta, mitochondrial | Q40089 | 78.5 | 15.4 | 3 | 0.39 |
| MDP0000416290 | Atpase 4, plasma membrane-type | Q9SU58 | 84.62 | 5.5 | 1 | 0.58 |
| ROS and NO signaling | ||||||
| MDP0000684170 | Heat shock 70 kda protein | Q9SKY8 | 69.76 | 3.3 | 1 | 1.54 |
| MDP0000697285 | Heat shock 70 kda protein | Q02028 | 79.23 | 12.4 | 1 | 1.64 |
| MDP0000319048 | Cationic peroxidase 1 | P22195 | 74.6 | 21.3 | 6 | 0.43 |
| MDP0000545323 | Peroxidase 42 | Q9SB81 | 83.55 | 8.4 | 1 | 0.47 |
| MDP0000243237 | Peroxidase 44 | Q93V93 | 56.11 | 33.5 | 7 | 0.35 |
| MDP0000208152 | Peroxidase 53 | Q42578 | 60.98 | 9.8 | 2 | 0.43 |
| MDP0000154541 | Peroxidase 53 | Q42578 | 63.5 | 13.2 | 1 | 0.66 |
| MDP0000192235 | Peroxidase 42 | Q9SB81 | 75.44 | 6.1 | 1 | 0.67 |
| MDP0000706473 | Peroxidase 53 | Q42578 | 63.61 | 17.4 | 2 | 0.67 |
| MDP0000283650 | Peroxidase 4 | A7NY33 | 62.46 | 7 | 2 | 0.62 |
| MDP0000136398 | Peroxidase 16 | Q96518 | 64.42 | 4.6 | 1 | 0.49 |
| MDP0000301828 | Peroxidase 53 | Q42578 | 79.22 | 12.7 | 2 | 0.49 |
| MDP0000209189 | Cationic peroxidase 1 | P22195 | 73.58 | 29 | 5 | 0.55 |
| MDP0000251955 | Mitogen-activated protein kinase | Q40353 | 86.38 | 5.9 | 2 | 1.51 |
| Protein homeostasis | ||||||
| MDP0000241084 | E3 ubiquitin-protein ligase RGLG1 | Q9SS90 | 71.97 | 6.7 | 1 | 1.51 |
| MDP0000317971 | E3 ubiquitin-protein ligase UPL2 | Q8H0T4 | 48.73 | 3.9 | 3 | 0.57 |
| MDP0000269081 | E3 ubiquitin-protein ligase RING1 | P0CH30 | 58.54 | 3.4 | 1 | 0.64 |
| MDP0000676693 | ATP-dependent 26S proteasome regulatory subunit | Q54DY9 | 34 | 22.7 | 4 | 2.03 |
| MDP0000322270 | ATP-dependent 26S proteasome regulatory subunit | Q7ZZ25 | 42.23 | 6.2 | 1 | 0.58 |
| MDP0000315993 | ATP-dependent Zn proteases | O80860 | 85.11 | 4.7 | 6 | 1.61 |
| MDP0000188831 | 20S proteasome, alpha and beta subunits | O82178 | 58.71 | 16.2 | 5 | 0.45 |
| MDP0000245541 | Ubiquitin carboxyl-terminal hydrolase 2 | Q8W4N3 | 51.36 | 0.9 | 1 | 0.45 |
| MDP0000283283 | Ubiquitin carboxyl-terminal hydrolase 24 | Q9FPS3 | 74.27 | 5.8 | 2 | 0.64 |
| MDP0000263256 | F-box protein | Q9FGY4 | 28.24 | 1.7 | 1 | 1.73 |
| MDP0000180936 | F-box protein | Q9FE83 | 54.18 | 4.9 | 1 | 2.38 |
| MDP0000141860 | 40S ribosomal protein S30 | P49689 | 98.39 | 16.1 | 1 | 0.38 |
| MDP0000265859 | 60S ribosomal protein L14-1 | Q9SIM4 | 87.72 | 14.7 | 2 | 0.53 |
| MDP0000865687 | 40S ribosomal protein S17-4 | Q9LZ17 | 83.69 | 17.9 | 1 | 0.39 |
| MDP0000544199 | 50S ribosomal protein L24 | A8LC45 | 49.51 | 10.9 | 1 | 0.39 |
| MDP0000283097 | 40S ribosomal protein S17-4 | Q9LZ17 | 79.43 | 7.3 | 1 | 0.45 |
| MDP0000266765 | 30S ribosomal protein S5 | P93014 | 68.2 | 9.5 | 3 | 0.64 |
| MDP0000772832 | 40S ribosomal protein S19-3 | Q9FNP8 | 78.06 | 25.2 | 2 | 0.58 |
| MDP0000417422 | 40S ribosomal protein S5 | O24111 | 90.24 | 18.7 | 1 | 0.59 |
| MDP0000169133 | Translation elongation factor | B7K735 | 62.8 | 2.6 | 2 | 0.44 |
| MDP0000903484 | Translation elongation factor EF-1 | P93447 | 62.83 | 21.5 | 2 | 0.44 |
| MDP0000800338 | Translation initiation factor IF-2 | P57997 | 70.64 | 5.5 | 1 | 0.62 |
| MDP0000142167 | Eukaryotic translation initiation factor 2 subunit | P55871 | 96.68 | 7.5 | 1 | 0.63 |
| MDP0000270113 | Eukaryotic translation initiation factor 3 subunit | Q40554 | 64.54 | 9.5 | 2 | 0.6 |
| MDP0000141898 | Eukaryotic translation initiation factor 3 subunit | Q38884 | 81.19 | 14.4 | 1 | 0.67 |
| MDP0000261642 | Tryptophanyl-tRNA ligase | Q5UPJ7 | 44.83 | 3.1 | 2 | 1.63 |
| MDP0000134153 | Histidine–tRNA ligase | P93422 | 33.14 | 2 | 1 | 1.61 |
| MDP0000770881 | Glycine–tRNA ligase 1 | O23627 | 58.43 | 8.5 | 1 | 1.56 |
| MDP0000207727 | Thiol-disulfide isomerase and thioredoxins | O80763 | 64.64 | 20.8 | 3 | 2.63 |
| MDP0000308890 | Thiol-disulfide isomerase and thioredoxins | O80763 | 62.36 | 15.5 | 3 | 2.39 |
| MDP0000297301 | Thiol-disulfide isomerase and thioredoxins | O80763 | 59.55 | 2.2 | 1 | 1.81 |
| MDP0000505556 | Cysteine protease | P25776 | 44.1 | 3.1 | 1 | 1.77 |
| Microtubules and Cell wall remolding | ||||||
| MDP0000812416 | Tubulin α-3 | P33627 | 96.05 | 18.3 | 2 | 1.58 |
| MDP0000282827 | α-tubulin suppressor | Q9P258 | 77.58 | 3.9 | 2 | 0.67 |
| MDP0000296747 | Xyloglucan endotransglucosylase/hydrolase | Q9F234 | 45.19 | 8.7 | 5 | 1.5 |
| MDP0000661960 | Xyloglucan endotransglucosylase/hydrolase | Q8LF99 | 78.36 | 14.4 | 2 | 2.7 |
| MDP0000640549 | Expansin-like protein | O23547 | 54.84 | 5.9 | 1 | 2.16 |
| MDP0000130769 | Pectin lyase-like superfamily protein | A7PZL3 | 52.81 | 12.5 | 4 | 1.53 |
| MDP0000248311 | Pectin lyase-like superfamily protein | P15922 | 26.05 | 5.2 | 3 | 1.72 |
| MDP0000943790 | Pectin lyase-like superfamily protein | Q949Z1 | 47.68 | 1.9 | 1 | 0.3 |
| MDP0000175757 | Pectin lyase-like superfamily protein | Q94AJ5 | 45.06 | 4.2 | 2 | 0.49 |
| MDP0000251956 | Pectin lyase-like superfamily protein | A7PZL3 | 80.32 | 17.4 | 4 | 0.49 |
| MDP0000753366 | Cellulase | P23548 | 24.41 | 4.8 | 1 | 2.32 |
| MDP0000055078 | α-l-arabinofuranosidase 1 | Q9SG80 | 70.21 | 16.8 | 5 | 1.57 |
a Accession no. is the locus name of a gene in apple genome; b SwissProt Accession is matched accession of blast in SwissProt database; c Identity means the score of blast in SwissProt database; d %COV (95) indicates the percentage of matching amino acids from identified peptides having confidence greater than or equal to 95%; e Mean ratio corresponds to protein reporter ion intensity originating from IBA-treated protein samples relative to control protein samples with a 1.5-fold change and a p < 0.05.