Figure 3.
Minoxidil upregulates expression of hair-growth promoting factors CXCL1, PD-ECGF, and PDGF-C in ASCs. (A) Relative mRNA expression levels of growth factors including CXCL1, PD-ECGF, and PDGF-C were measured in minoxidil-treated ASCs; (B–D) Human recombinant CXCL1, PD-ECGF, and PDGF-C proteins each facilitated hair growth and increased the number of mature hair follicle after injection into the dorsal skin of shaved mice as revealed by hair weight at 2 weeks post-injection. n = 6–8 mice per group. * p < 0.05, ** p < 0.01, *** p < 0.001. (E) CXCL1, PD-ECGF, and PDGF-C proteins each facilitate mouse vibrissal hair follicle growth ex vivo. ** p < 0.01. n = 10 samples per treatment group. All error bars indicate SEM.