Table 2.

Description of primary cell lines derived from OS samples (OSDC) and from bone marrow (MSC).

Patient’s OSDC	1	2	3	4	5	6	7	8	9
Time of tumor sample collection for primary cell lines	post-NAPC	post-NAPC	post-NAPC	post-NAPC	pre-NAPC	post-NAPC	post-NAPC	post-NAPC	post-NAPC
Sample storage before dissociation	in culture medium P/S at 4 °C for 12 h			in SVF with 10% DMSO at −80 °C for 1 to 4 weeks			in MACS Tissue Storage Solution at 4 °C for 24 to 72 h
Enzymatic dissociation	No	in Collagenase I (InVitrogen) during 1 to 4 h at 37 °C					in MACS human tumor dissociation kit (Miltenyi Biotech) for 1 h at 37 °C
Sphere rate (10−5)	n.d.	65	0	3.3	0.67	5.5	15.8	n.d.	47
Patient’s MSC	n.a.	2	3	4	5	6	7	n.a.	n.a.
Time of BM aspirates	n.a.	pre-NAPC	post-NAPC	post-NAPC	pre-NAPC	pre-NAPC	pre-NAPC	n.a.	n.a.
Sphere rate (10−5)	n.a.	0	0.13	n.a.	n.a.	0	0	n.a.	n.a.

Adherent cells were derived from OS samples (OSDC) or from bone marrow (MSC) when available (not available n.a.). Tissue samples were collected before or after neo-adjuvant poly-chemotherapy (pre- or post-NAPC). Sample storage conditions and dissociation method are indicated. Sphere rates obtained following anchorage-independent conditions are indicated when determined. Not determined (n.d.). Not available (n.a.).