Figure 2.

Overexpression of Bax inhibitor-1 (BI-1) inhibits virus induced cell death and cell cycle arrest in MDCK cells. (A) Control mock-infected MDCK, BI-1-overexpressing MDCK, and BI-1 ΔC-overexpressing MDCK cells were seeded in 96-well cell culture plates and infected with A/PR/8/34 virus at 100 TCID₅₀. The media were changed 2 h after virus infection, and MTT assays were performed 24 h and 48 h after virus infection. Data were shown as the relative expression of cell metabolic activity of BI-1-overexpressing MDCK, and BI-1 ΔC-overexpressing MDCK cells compared to the control infection from three individual experiments (mean ± SEM) (* p < 0.05). (B) Control MDCK, BI-1-overexpressing MDCK, and BI-1 ΔC-overexpressing MDCK cells were seeded in 6-well cell culture plates and infected with A/PR/8/34 virus at 1000 TCID₅₀. Anti-influenza activity was determined three times calculating the apoptotic sub-G0/G1 populations by flow cytometric analysis 24 h, 36 h, and 48 h after virus infection. Data were expressed as relative numbers of apoptotic sub-G0/G1 populations of BI-1-overexpressing MDCK, and BI-1 ΔC-overexpressing MDCK cells compared to the control (mean ± SEM) (* p < 0.05, ** p < 0.01).