Figure 2.

Intraperitoneal injection of SK-216 suppresses lung metastasis of 143B cells in a mouse model. (a) 143B-Luc cells were inoculated into the right knee of a model mouse. Lung metastases at 5 weeks after inoculation are reflected in bioluminescence; (b) the areas of metastatic lesion on the lung in the model mice were plotted. The black bar indicates the mean value. Representative hematoxylin and eosin (H & E) staining of the lung at five weeks after inoculation are shown. Scale bars, 500 μm; (c) Primary tumors at five weeks after cell-inoculation are reflected in bioluminescence. Total Flux (photons/seconds) measured in the obtained IVIS images of mice. The black bar indicates the mean value; (d) the rates of tumor cells that were positive for Ki-67 in primary tumors were calculated by counting 10 visual fields at high magnification. Representative staining of Ki-67 from control and SK-216 treated mice are shown. Scale bars, 50 μm.