Figure 6.

Relative quantification of marker genes and Wnt5a after treatment with nicotine and mecamylamine. (A,B) Relative quantification of marker genes after treatment with nicotine for three days and seven days; (C,D) relative quantification of Wnt5a after treatment with nicotine for three days and seven days; (E) relative quantification of Wnt5a after treatment with mecamylamine for seven days; (A–E) the results are based on three independent experiments and are expressed as mean values ± SD. An asterisk indicates a statistically significant difference from untreated control organoids (Student’s t-test, * p < 0.05).