Figure 8.
Rescue of organoid growth and differentiation after co-treatment with mecamylamine and recombinant Wnt5a. (A) Micrographs of organoids after treatment with 10−4 M mecamylamine and 10−4 M mecamylamine/Wnt5a for seven days; (B) effect of 10−4 M mecamylamine and 10−4 M mecamylamine/Wnt5a on the size of cultured organoids. Each sample represents an average of three independent experiments. Error bars represent the SD of the mean. An asterisk indicates a statistically significant difference from untreated control organoids (Mann–Whitney test, n.s., not significant, ** p < 0.005, and *** p < 0.0005). (C) Effect of 10−4 M mecamylamine and 10−4 M mecamylamine/Wnt5a on the expression of ISC markers and epithelium in a seven-day culture of organoids; (D) relative quantification of marker genes after treatment with 10−4 M mecamylamine and 10−4 M mecamylamine/Wnt5a for seven days. The results are based on three independent experiments and are expressed as mean values ± SD. An asterisk indicates a statistically significant difference from untreated control organoids (Student’s t-test, * p < 0.05).