Table 2.
Differential expression patterns of marker genes and genes expressed in the Wnt pathway and other stem cell pathways, after treatment with nicotine and mecamylamine.
| Accession | Gene Name | Nicotine (Fold Change *) |
Mecamylamine (Fold Change *) |
|||
|---|---|---|---|---|---|---|
| 10−7 M(3 Day) |
10−5 M(3 Day) |
10−7 M(7 Day) |
10−5 M(7 Day) |
10−6 M (7 Days) |
||
| NM_019827 | Gsk3β | 1.09 | 1.05 | 1.64 | 1.71 | 1.00 |
| NM_007614 | Ctnnb1 | 0.91 | 0.78 | 1.61 | 1.88 | 0.84 |
| NM_007561 | Bmpr2 | 1.01 | 1.06 | 1.60 | 1.70 | 0.98 |
| NM_007889 | Dvl3 | 1.08 | 1.02 | 1.74 | 1.73 | 1.10 |
| NM_001005784 | Patj | 0.39 | 0.62 | 3.74 | 3.88 | 1.06 |
| NM_026735 | Mob1b | 1.15 | 1.10 | 2.27 | 2.37 | 0.83 |
| NM_007862 | Dlg1 | 1.19 | 1.18 | 1.74 | 1.96 | 1.66 |
| NM_001109752 | Dlg4 | 16.37 | 7.20 | 2.28 | 2.62 | 0.80 |
| NM_145571 | Mob1a | 1.04 | 1.05 | 1.64 | 1.84 | 0.96 |
| NM_001166585 | Tead1 | 1.65 | 1.30 | 1.95 | 1.64 | 1.13 |
| NM_010690 | Lats1 | 1.10 | 1.08 | 1.64 | 1.62 | 0.98 |
| NM_001164112 | Nfatc1 | 1.20 | 0.87 | 1.60 | 1.99 | 0.00 |
| NM_009521 | Wnt3 | 1.10 | 1.13 | 1.10 | 1.03 | 1.05 |
| NM_001256224 | Wnt5a | 1.30 | 1.37 | 4624.5 | 3885.7 | 0.00 |
| NM_011719 | Wnt9b | 1.30 | 1.25 | 1.84 | 1.41 | 0.29 |
| NM_020510 | Frizzled-2 | 1.16 | 1.14 | 1.11 | 0.95 | 0.89 |
| NM_008055 | Frizzled-4 | 0.98 | 0.94 | 1.82 | 1.99 | 0.83 |
| NM_022721 | Frizzled-5 | 1.05 | 0.89 | 1.84 | 1.80 | 0.97 |
| NM_001162494 | Frizzled-6 | 1.02 | 1.08 | 2.01 | 1.88 | 1.02 |
| NM_010195 | Lgr5 | 1.16 | 1.09 | 1.46 | 1.78 | 0.53 |
| NM_011448 | Sox9 | 1.07 | 1.33 | 1.25 | 1.48 | 0.27 |
| NM_009719 | Ngn3 | 1.30 | 1.59 | 0.97 | 1.39 | 0.28 |
| NM_009327 | Hnf1 | 1.10 | 1.17 | 1.19 | 1.31 | 0.15 |
| NM_010637 | Klf4 | 1.03 | 1.04 | 1.31 | 1.44 | 0.17 |
* Fold change is defined as the ratio of the signal of non-treated organoids to that of treated organoids.