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. 2018 Mar 7;19(3):762. doi: 10.3390/ijms19030762

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Functional analysis of selected asRNAs. (A) Binding position of sequence-analysed individual asRNAs, expressed from the pcDNA 4.0 plasmid, within the KRT14 target region spanning from exon 5 to intron 7; (B) flow cytometric analysis of HEK293 cells upon triple-transfection with the KRT14-scRTM, the KRT14-scMG and individual asRNAs increased the trans-splicing efficiency in comparison to that detectable in HEK293 cells transfected with the screening molecules and an empty (without asRNA sequence) pcDNA4.0 expression plasmid in all transfection experiments. The level of accurate trans-splicing was quantified by flow cytometric counting of GFP-expressing cells two days post treatment and referred to the pcDNA4.0 control (set to 1). Two independent transfection experiments (HEK1 and HEK2) are shown.