Table 1.
Methods for iPSC generation and quality control in BD studies.
| Ref. | Source | Factors | Methods | N° | Auth | Karyo | Pluripotency |
|---|---|---|---|---|---|---|---|
| [63] | FB | OKSM | RV | ≥5 | nd | nd | ICC, EB and TriL |
| [64] | FB | miR/NAM | LV | na | na | na | ICC |
| [65] | FB | OKSM | RV | ≥3 | SNP | G-Band | ICC, PluriTest, EB and ScoC, Tera |
| [66] | FB | OKSM | SV | 2 | nd | G-Band | ICC, TriL |
| [67] | FB | miR/NAM | LV | na | na | na | ICC |
| [68] | FB | OKSM | SV | 3 | nd | G-Band | ICC, EB and TriL |
| [69] | LCL | OKSM, LIN28 | Epi | ≥3 | STRP | nd | ICC |
| [70] | FB, LB | OKSM | Epi, LV, RV | 1–3 | SNP | nd | ICC, PluriTest, EB, Tera |
| [71] | AP | OKSM | Epi | 1 | nd | nd | ICC, EB and TriL |
AP, adipocytes; Auth, authentication; EB, undirected embryoid body formation; Epi, episomal plasmid; FB, fibroblast; G-Band, chromosomal G-banding; ICC, immunocytochemistry; Karyo, karyotype; LB, lymphoblast from blood; LCL, lymphoblastoid cell line; LV, lentiviral transduction; miR/NAM, miR9/9*-124, NEUROD2, ASCL1, and MYT1L; OKSM, OCT4, KLF4, SOX2, MYC; N°, numbers of independent clones per donor; na, not applicable; nd, not determined; PluriTest, a bioinformatic approach to asses pluripotency [72]; Tera, teratoma formation; TriL, analysis of trilineage formation by ICC and/or qRT-PCR; Ref, reference; RV, retroviral transduction; ScoC, lineage score card [73]; SNP, whole genome single nucleotide profiling; SV, Sendai virus transduction; STRP, short tandem repeat profile.