Figure 7.

miR-146a-5p did not target IRS1 gene (A) miR-146a-5p is different from miR-146b at the 18th and the 21th base, and there is one more base at the end of miR-146a-5p. We therefore synthesized three mutants. For mut1, the 18th base A was replaced by G; for mut2, the 21th base C was replaced by U; for mut3, both the 18th and the 21th base were replaced according to the sequence the miR-146a-5p. (B) Western blot and gray-scale scanning analyses of IRS1 after transfection of miR-146a-5p mimics/NC/inhibitor/iNC (n = 4); (C) pmirGLO dual-luciferase reporter vectors analysis between miR-146a-5p and the 3′UTR sequences of IRS1 gene.