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. 2018 Mar 10;19(3):800. doi: 10.3390/ijms19030800

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A,B) Effect of a graded concentration of Astragalus membranaceus extract (5–100 μg/mL) in the presence of LPS + IFN on reactive oxygen species (ROS) levels, detected by 2′,7′ dichlorofluorescein-diacetate (H₂DCF-DA). (C,D) Effect of Astragalus membranaceus extract (50 μg/mL) in the presence of H₂O₂ on ROS formation, evaluated with the probe H₂DCF-DA. Data are expressed as mean ± SEM. ***, ** denote p < 0.001 and p < 0.01 vs. LPS + IFN or vs. H₂O₂.

(A,B) Effect of a graded concentration of Astragalus membranaceus extract (5–100 μg/mL) in the presence of LPS + IFN on reactive oxygen species (ROS) levels, detected by 2′,7′ dichlorofluorescein-diacetate (H₂DCF-DA). (C,D) Effect of Astragalus membranaceus extract (50 μg/mL) in the presence of H₂O₂ on ROS formation, evaluated with the probe H₂DCF-DA. Data are expressed as mean ± SEM. ***, ** denote p < 0.001 and p < 0.01 vs. LPS + IFN or vs. H₂O₂.