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. 2018 Mar 11;19(3):809. doi: 10.3390/ijms19030809

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cellular localization of ExtI. (A) Subcellular fractionation and Western blotting. Each cellular fraction was isolated, and the localization of ExtI was analyzed by Western blotting (upper panel). A Coomassie-stained gel is shown as the loading control (lower panel). The numbers at the left indicates the sizes of the marker proteins in kDa; (B) the effects of proteinase K treatment on ExtI integrity. Cells were treated with different concentrations (0 to 30 mg/mL) of proteinase K to analyze the surface exposure of ExtI, and the degraded products were analyzed by Western blotting.