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. 2018 Mar 13;19(3):841. doi: 10.3390/ijms19030841

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of PCB118, PCB153 and BaP in “Time 1” conditions on the expression of genes related to adipocyte differentiation, glucose and lipid homeostasis, and inflammation. 3T3-L1 cells were incubated with or without PCB118 (one or five micromolars) or PCB153 (one or five micromolars) in the presence or not of BaP (one micromolar) according to the “Time 1” conditions described in materials and methods. Cells were washed twice with PBS and mRNA extraction and qRT-PCR were performed as described under materials and methods. Each incubation condition was carried out in quadriplicate and the experiment was repeated three times with similar results. The data are mean ± SD. The level of significance is given by comparison of treated trials with controls (n = 4 per group): * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Effects of PCB118, PCB153 and BaP in “Time 1” conditions on the expression of genes related to adipocyte differentiation, glucose and lipid homeostasis, and inflammation. 3T3-L1 cells were incubated with or without PCB118 (one or five micromolars) or PCB153 (one or five micromolars) in the presence or not of BaP (one micromolar) according to the “Time 1” conditions described in materials and methods. Cells were washed twice with PBS and mRNA extraction and qRT-PCR were performed as described under materials and methods. Each incubation condition was carried out in quadriplicate and the experiment was repeated three times with similar results. The data are mean ± SD. The level of significance is given by comparison of treated trials with controls (n = 4 per group): * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.