Figure A2.

Effects of PCB118, PCB153 and BaP on undifferentiated 3T3-L1 cells on the expression of various adipogenesis related genes. Undifferentiated 3T3-L1 cells were incubated with or without PCB118 (one or five micromolars) or PCB153 (one or five micromolars) in the presence or not of BaP (one micromolar). Cells were washed twice with PBS and mRNA extraction and qRT-PCR were performed as described under materials and methods. Each incubation condition was carried out in quadriplicate and the experiment was repeated three times with similar results. The data are mean ± SD. The level of significance is given by comparison of treated trials with controls (n = 4 per group): * p < 0.05; ** p < 0.01.