Table 2.
Designed primers used for different gene amplifications.
| Gene | Primer Sequence 5′→3′ | Amplified Fragment (bp) |
|---|---|---|
| ESF1 | FW: GCTCCTCGTGCTGATGAGATTA | 176 |
| RV: TGCTCTTCCTTCATCCTCTCCT | ||
| PCM1 | FW: TCAAGACAAGAAAAGCGTCTGC | 180 |
| RV: GGGCTGAATGTCTGTTCCTACT | ||
| TPR | FW: TTTGGCACAGTTTCGGCTAC | 164 |
| RV: TCTTCCTCAGTTCCTACAGGTG | ||
| RIF1 | FW: TAATAAGGTTCGCCGTGTCTCC | 177 |
| RV: CCTTTGGCTGAAGTGGTATTATGC | ||
| REV3L | FW: TGAGAAATGAGGTGGCTCTAAC | 168 |
| RV: CACGGACACGGCTAACATAA | ||
| RAD50 | FW: GCCTCACTCATCATTCGCCT | 168 |
| RV: AAGCTGGAAGTTACGCTGCT | ||
| ROCK1 | FW: ATGGAACCAGTACAACAAGCTGA | 159 |
| RV: GCATCTTCGACACTCTAGGGC | ||
| ROCK2 | FW: GAAGAGCAGCAGAAGTGGGT | 170 |
| RV: GGCAGTTAGCTAGGTTTGTTTGG | ||
| CCDC91 | FW: AAGTCAGGAAACTGTTAAGGCAG | 152 |
| RV: ACAGGCTTCTTTGGCGGAT | ||
| DYNC2H1 | FW: GCTTGGCGGAGCAGATTAAA | 159 |
| RV: CCAGGATGCCCGATTCAGTAT | ||
| 18S | FW: AGTGAAACTGCAATGGCTC | 167 |
| RV: CTGACCGGGTTGGTTTTGAT |
Note: FW = forward primer; RV = reverse primer. Official full names of the genes are shown in Table 1.