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. 2018 Mar 15;19(3):875. doi: 10.3390/ijms19030875

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Preparation and purification of EoblGOBP2 protein analyzed by SDS-PAGE. M: protein molecular weight marker. Lane 1: the crude bacterial extracts before induction by IPTG; lane 2: the supernatant of crude bacterial extracts after the induction; lane 3: the purified EoblGOBP2 with His-tag; lane 4: the final purified EoblGOBP2 protein digested by enterokinase.