Table 1.

Dissociation constants (μM) of EoblGOBP2 and tea volatiles with the fluorescent probe 1-NPN.

Chemical Category	Tea Volatiles	[IC50] (μM)	KD (μM) Dissociation Constant	Percentage in the Total Tea Leaves Volatiles (%)
Esters	Dibutyl phthalate	19.42	4.35	0.08
	Methyl salicylate	194.05	43.51	2.37
	Triethyl phosphate	―	―	0.53
Ketones	β-ionone	56.13	12.59	0.11
	Acetophenone	57.86	12.97	0.31
Aldehydes	(E)-2-decenal	92.41	20.72	0.23
	(E)-2-hexenal	143.08	32.24	0.46
	Benzaldehyde	197.20	44.21	0.13
	(E)-2-pentenal	―	―	0
Alcohols	(Z)-3-hexenol	―	―	0
	n-pentanol	―	―	0.45
	Benzyl alcohol	―	―	0
	n-hexanol	―	―	0.06
	1-penten-3-ol	―	―	0.09
	α-terpineol	―	―	0.31
	Linalool	―	―	0
	Nerol	―	―	0.200
Others	Hexadecanoic acid	―	―	0.251
	1,3-dioxolane	―	―	0.435

Solution of protein was at 1.5 μM with and the added concentration of 1-NPN was in line with the dissociation constants of EoblGOBP2/1-NPN complex calculated. Dissociation constants (K_D) were calculated from the values of ligands halving the 1-NPN fluorescence (IC₅₀), as described in the methods. Dissociation constants of ligands whose IC₅₀ exceeded 50 mM are represented as ‘‘―’’.