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. 2018 Mar 16;19(3):877. doi: 10.3390/ijms19030877

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Changes in cauliflower leaf chlorophyll content minimal (Fo), maximal (Fm) and variable (Fv) fluorescence and Fv/Fm ratio in control grown (K), cold-stressed (C), heat-stressed (H), cold-recovered (CA) and heat-recovered (HA) plants. Chlorophyll content measured with a chlorophyll meter was expressed in relative units. Chlorophyll fluorescence was measured using a portable fluorometer. Before measurement, leaves were dark adapted for 30 min. Photochemical efficiency of PSII could be estimated from the Fv/Fm ratio, where Fv is the difference between Fm and Fo. Bars are means ± SD (n > 3) and asterisks indicate significant differences (p < 0.05; Student’s t-test) from the control (K). Further experimental details in ‘Materials and Methods’.