Figure 2.

Total tau protein was increased by L-homocysteine (L-Hcy) in M1C cells. M1C cells were subjected to 5 days of tau expression (by reducing tetracycline) and cells were exposed to 10, 100, 1000 µM L-Hcy during the final 24 h of tau induction. The amount of Tau5 positive tau was increased in a dose-dependent manner when cells were treated with L-Hcy. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a loading control. Bar: ± SD, ** p < 0.01 (A,B). qPCR showed that mRNA levels of tau did not change with Hcy treatment (C). NI: non induced